rabbit anti mouse ifitm3 antibody  (Proteintech)

Journal: Journal of Neuroinflammation

Article Title: Neuronal TDP-43 pathology drives astrocytic interferon response in a mouse model of ALS

doi: 10.1186/s12974-025-03658-2

Figure Lengend Snippet: Protein expression of ISG in rNLS8 brains. A . Expression of IFIT3 and ISG15 protein at different time points after induction of disease in rNLS8 mice (source https://shiny.rcc.uq.edu.au/TDP-map . B . Representative examples of immunohistochemical (IHC) staining in the region of the hippocampus of control and rNLS8 mice with the antibodies against the antigens indicated. Wild type (control) mouse on top row; rNLS8 mouse bottom row. Left column: brown indicates positive staining for ISG15; blue, hematoxylin counterstain. Original magnification 20x; bar = 100 microns. Right column: brown indicates positive staining for IFITM3, blue, hematoxylin counterstain. Original magnification 20x, bar = 100 microns. Inset bars = 50 microns. “n” = neurons of the hippocampus. C . Semi-quantitative scoring of IFITM3 and ISG15 in the brains of control and rNLS mice (n=4 per group, scores for individual mice are represented by circles [control mice] and boxes [rNLS8] mice on the graph). Y-axis is the semi-quantitative score from 0–4 (see Methods). P -values were calculated using the unpaired nonparametric Mann-Whitney t-test (GraphPad Prism 10 Software) (* indicates p < 0.05) D . Three color immunofluorescence of formalin-fixed paraffin-embedded tissues from control and rNLS8 mouse in the region of the hippocampus was performed as described in Methods. Bar = 100 microns. Red indicates NeuN expression (neurons); green indicates GFAP expression (astrocytes); yellow indicates ISG15 expression; blue indicates DAPI expression (nuclei). White arrows mark regions of ISG15 expression associated with GFAP expression. Inset also shows co-localizing astrocyte (green) and ISG15 (yellow) expression. E . Upper panel. Quantitative analysis of ISG15 expression in the region of the hippocampus in control and rNLS8 mice ( n =4 per group), Y-axis is the percent positive ISG15 expression (see Methods). P -values were calculated using the unpaired nonparametric Mann-Whitney t-test (GraphPad Prism 10 Software) (* indicates p < 0.05). Lower panel. Quantitative analysis of the percentage of ISG15 within GFAP in the region of the hippocampus in control and rNLS8 mice

Article Snippet: The following primary polyclonal antibodies were used: rabbit anti-mouse IFITM3 antibody, clone RB13975 (Antibodies online, Limerick, PA, catalog # ABIN2845007) at 1:1,000; rabbit anti-mouse ISG15 antibody (Proteintech, Rosemont, IL, catalog #15981–1-AP) at 1:300.

Techniques: Expressing, Immunohistochemical staining, Immunohistochemistry, Control, Staining, MANN-WHITNEY, Software, Immunofluorescence, Formalin-fixed Paraffin-Embedded

Journal: The EMBO journal

Article Title: Interferon-inducible phospholipids govern IFITM3-dependent endosomal antiviral immunity.

doi: 10.15252/embj.2022112234

Figure Lengend Snippet: Figure 1. Endosomal and PM-bound IFITM3 restrict viral entry and infection through distinct mechanisms.

Article Snippet: Ten microgram of rabbit anti-IFITM3 Ab (from proteintech Cat# 11714- 1-AP) or anti-mouse IgG control were added to the beads and left in incubation for an O/N with rotation.

Techniques: Infection

Journal: The EMBO journal

Article Title: Interferon-inducible phospholipids govern IFITM3-dependent endosomal antiviral immunity.

doi: 10.15252/embj.2022112234

Figure Lengend Snippet: Figure 7. IFITM1 and plasma membrane mutants IFITM3 inhibit SARS CoV2 entry and infection.

Article Snippet: Ten microgram of rabbit anti-IFITM3 Ab (from proteintech Cat# 11714- 1-AP) or anti-mouse IgG control were added to the beads and left in incubation for an O/N with rotation.

Techniques: Clinical Proteomics, Membrane, Infection

Journal: Journal of Virology

Article Title: The Interferon-Stimulated Gene Ifitm3 Restricts West Nile Virus Infection and Pathogenesis

doi: 10.1128/jvi.00581-16

Figure Lengend Snippet: Figure 4. Ifitm3-/- mice have normal levels of immune cell populations in the brain 725

Article Snippet: A 1:1,000 dilution of mouse anti-β-actin (Protein Tech 11714-1-AP) and rabbit anti-191 Ifitm3 (Cell Signaling 3700) was prepared in TBS-T + 4% milk and used to stain the membrane 192 overnight at room temperature.

Techniques: